The main objective of serial dilution is to reduce the concentration of a substance, typically a chemical or biological sample, in a series of steps. Serial dilution only reduces the number of bacteria/viable cells but doesn’t separate them like in other techniques like Flow Cytometry. This makes it easier to calculate the cell numbers in the primary solution by calculating the total dilution over the whole series. In serial dilution, the cell count or density gradually decreases as the serial number increases in each step. In the laboratory, this method is used to decrease the counts of viable cells within a culture to simplify the operation. In a single and very simple word, Serial dilution is a laboratory technique, in which a stepwise dilution process is performed on a solution with an associated dilution factor. Serial dilution involves performing a series of sequential dilution steps to convert a dense solution to a more usable concentration. Hey there, you might be thinking, what is serial dilution?Īs the term indicates, it is a series of succeeding dilutions that performed to create a less dense or less concentrated solution from a high dense or concentrated solution. This methodology is particular for bacterial counts (CFUs), but can be adapted for fungus (CFUs) and viruses (plaque-forming units, PFUs for viral counts).Īlso Read: MCQ on Serial Dilution What is a serial dilution? – serial dilution definition The objective may be to determine bacterial, fungal, or viral numbers. Following is a step-by-step process for solving dilution problems, followed by some practise problems. Most samples include an excessive number of microorganisms, necessitating successive dilution for accurate quantification. coli suspensions in nutritional broth, soil samples, and hamburger. It is routine practise to quantify microbial counts for both liquid and solid specimens, including E.
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